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steT [2019-02-24 17:45:42]
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steT [2019-02-24 17:45:42]

serine/ threonine exchanger transporter
Locus
BSU12860
Isoelectric point
9.18
Molecular weight
46.99 kDa
Protein length
438 aa Sequence Blast
Gene length
1317 bp Sequence Blast
Function
exchange of serine and threonine
Product
serine/ threonine exchanger transporter
Essential
no
Synonyms
ykbA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Transporters/ other → Amino acid transporters → APC superfamily
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    1,351,375 → 1,352,691

    The protein

    Protein family

  • amino acid-polyamine-organocation (APC) superfamily (according to UniProt)

    • Structure

  • 6F34 (from Geobacillus kaustophilus, 25% identity) PubMed

    • Localization

  • membrane

    • Expression and Regulation

    Operons

    Genes
    steT
    Description
    PubMed

    Regulation

  • expression activated by glucose (4.2 fold) PubMed
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    Biological materials

    Mutant

  • GP2378 ∆steT ::cat, available in Jörg Stülke's lab
  • GP2945 ∆steT ::kan, available in Jörg Stülke's lab
  • MGNA-A736 (ykbA::erm), available at the NBRP B. subtilis, Japan
  • BKE12860 (ΔsteT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCCTTAACCTCCTATG, downstream forward: _UP4_TGATAAAACGGTTCCCTTGT
  • BKK12860 (ΔsteT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCCTTAACCTCCTATG, downstream forward: _UP4_TGATAAAACGGTTCCCTTGT

    • Expression vector

  • pGP2281: expression of steT by pBQ200 in B. subtilis, available in Jörg Stülke's lab

    • LacZ fusion

  • pGP2275 (in pAC5) (GP2962), available in Jörg Stülke's lab

    • References

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